We firmly believe that science is about sharing resources and knowledge!
If you are interested in working with us or would like to get access to any of our equiptment/resources please contact us. We are always happy to share our resources, give advise on behavioural testing, or start new colaborations.
Access to equiptment is free (consumables excluded), however contribution to running costs/maintanence would be appreciated from permanent users.
Software/Algorithms developed/implemented in the laboratory can be accessed on our GitLab page.
Automated analysis of behavioural tests using ML
Use on request.
We have two systems for the detection of calcium-related fluctuations in cellular activity:
1.) Doric fiber photometry for the detection of population (bulk) signal.
2.) Inscipix miniature microscopes (GRIN lenses) for the detection of activity from individual neurons.
We have validated several constructs for the detection of general activity markers (e.g. GCaMP6g) or neurotransmitter sensors (e.g. dLight1.2). We offer access to this technology to interestested groups via our Special Interest Group: Brain Circuit Analysis.
Operant Boxes & Behavioural assessment
We have set up a bank of 8 modular operant chambers including a setup for optogenetic stimulation for the assessment of motor, cognitive, and psychiatric phenotypes. We have set up a series of tests to measure attention, neglect, short term memory, reaction and movement times. Our lab is well equipt for phenotyping transgenic and toxin based models of neurodegenerative disorders as well as detecting small improvements by therapeutical interventions.
The operant boxes were partly funded by the Parkinsonsfoundation and the Segerfalk foundation.
We us carbon fiber electrods and/or microelectrode arrays to measurea analytes of interest in vitro and in vivo. In our lab we have set up electrochemical detection for Dopamine, Glutamate, and GABA. Additional neurotransmitter systems that can be analysed but have not been used by our laboratory can be found here.
Electrochemistry allows for the sub-second recordings of neurotransmitters in acute and chronic recordings. Our system is fully optogenetics compatible and we can perform recordings in vitro, anaesthetised, and freely moving rodents. Should you be iterested in using the equiptment, collaborations, or want more information on the technology please contact us.
Inverted microscope: Leica DMI8
We have a Leica DMI8 inverted microscope fitted with cubes for DAPI, Cy2, Cy3, and Cy5 and the following objectives: x5, x10, x20 and x63. The microscope has an automated high precision stage and is great for overviews as well as close up pictures. We have both, a monochrome camera for flourescence and a full colour camera for brightfield images.
As can be seen in the sample images below we can perfom darkfield microscopy and generate EDF images.
We have an ImageJ macro for offline stereology which works really well on overviews taken at higher magnification. If you are interested inusing the microscope email us for an introduction.
Our Leica inverted Microscope was funded by the Crafoord foundation and the Kungl- Fysiografiska Sällskapet i Lund.
It is currently used by 7 different research groups.
We have a Sutter P1000 Micropipette puller, Narishige Micropipette grinder, and a Narishige Microforge for the production of glass capillaires. These can be used for injections into the brain or for the production of carbon fibre electrodes for electrochemical recordings. If you would like access to the equiptment or want glass capillaries for your experiments contact us.
Our Micropipette puller has been financed by a grant from the Parkinsonsfoundation.
It is currently used by 12 different groups.
We have a blue and a yellow laser including a MASTER8 controller for optogenetic stimulation. The setup is movable and has been used with our operant boxes, freely moving recordings, electrochemistry in vivo and cell culture electrochemistry recordings.
We are managing the Trophos platerunner platform, which enables the rapid acquisition of full well images from 96/384 well plates at high resolution.
UV - 360/370nm (50mW)
Blue - 455/490nm (100mW)
Green - 505/555nm (50mW)
Orange - 587/595nm
Red - 620/645nm
High pass filter > 455nm (DAPI, Hoechst)
Band pass filter 495/540nm (AF488, FITC)
High pass filter > 510nm (Cy2)
Band pass filter 570/620nm (DsRed, YFP, Cy3)
Band pass filter 618/675nm (mCherry)
High pass filter > 665nm (Cy5)
We have a workstation for Machine Learning (ML) applications or other image manipulations requiring high computing power.
Our ASUS Strix G35DX NR002W is fitted with a Ryzen9 processor, 32GB RAM and a RTX 3090 graphics card.