BNL Lab members are boldend


Rifes P, Kajtez J, Rågåard Christiansen J, Schörling A, Singh Rathore G, Wolf DA, Heuer A, Kirkeby A: Forced LMX1A expression induces dorsal neural fates and disrupts patterning of human embryonic stem cells into ventral midgrain dopaminergic neurons. Stem Cell Reports. 2024.


Anwer DM*, Gubinelli F*, Kurt YA, Sarauskyte L, Jacobs F, Venuti C, Sandoval IM, Yang Y, Stancati J, Mazzocchi M, Brandi E, O'Keeffe G, Steece-Collier K, Li J-Y, Deierborg T, Manfredsson FP, Davidsson M, Heuer A: A comparison of machine learning approaches for the quantification of microglial cells in the brain of mice, rats and non-human primates. Plos One. 2023.

* Joined 1st author

Rajova J, Davidsson M, Hartnor M, Aldrin-Kirk P, Cardoso T, Nolbrant S, Mollbrink A, Storm P, Heuer A, Parmar M, Björklund T: Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. Molecular Therapy - Methods & Clinical Development. 2023.

Canals I, Comella-Bolla A, Cepeda-Prado E, Avaliani N, Crowe JA, Oburoglu L, Bruzelius A, King N, Pajares MA, Perez-Sala D, Heuer A, Rylander-Ottosson D, Soriano J, Ahlenius H: Astrocyte dysfunction and neuronal network hyperactivity in a CRISPR engineered pluripotent stem cell model of frontotemporal dementia. Brain Communications. 2023.

Gubinelli F, Sarauskyte L, Venuti C, Kulacz I, Cazzolla G, Negrini M, Anwer D, Vecchio I, Jacobs F, Manfredsson FP, Davidsson M, Heuer A: Characterisation of functional deficits induced by AAV overexpression of alpha-synuclein in rats. Current Research in Neurobiology 4, 100065. 2023.


Quintino L*, Gubinelli F*, Arvidsson E, Davidsson M, Lundberg C, Heuer A: Automated quantification of neuronal swellings in a preclinical rodent model of Parkinson’s disease detects region-specific changes in pathology. Journal of Neuroscience Methods. 2022. doi: 10.1016/j.jneumeth.2022.109640

* Joined 1st author

Gubinelli F, Cazzolla G, Negrini M, Kulacz I, Mehrdadian A, Tomasello G, Sarauskyte L, Venuti C, Jacobs F, Manfredsson FP, Davidsson M, Heuer A: Lateralized deficits after unilateral AAV-vector based overexpression of alphasynuclein in the midbrain of rats on drug-free behavioural tests. Behavioural Brain Research. 2022.

Negrini M, Tomasello G, Davidsson M, Fenyi A, Adant C, Hauser S, Espa E, Gubinelli F, Manfredsson FP, Melki R, Heuer A: Sequential or simultaneous injection of preformed fibrils and AAV overexpression of alpha-synuclein are equipotent in producing relevant pathology and behavioural deficits.

Journal of Parkinson's Disease. 2022.

Aldrin-Krik P, Akerblom M, Cardoso T, Nolbrant S, Adler A, Liu X, Heuer A, Davidsson M, Parmar M, Björklund T: A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants. Stem Cell Reports. 2021. 10.1016/j.stemcr.2021.11.014

Davidsson M, Heuer A: Small scale adeno-associated virus-vector prodicion for preclinical gene delivery based on chloroform precipitation. Neural Regeneration Research 17(1):99-100.


Heuer A: Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods.Methods in Molecular Biology 2352:201-226. 10.1007/978-1-0716-1601-7_14

Rylander Ottosson D, Grade S, and Heuer A: Editorial: Regeneration and Brain Repair. Frontiers in Cellular Neuroscience, (2021). doi: 10.3389/fncel.2021.687992

Frontiers special issue edited:


Davidsson M, Negrini M, Hauser S, Svanbergsson A, Lockowandt M, Tomasello G, Manfredsson FP, Heuer AA comparison of AAV-vector production methods for gene therapy and preclinical assessment. Scientific Reports, (2020) 10:21532.

Negrini M, Wang G, Heuer A, Björklund T, & Davidsson M: AAV production everywhere: A simple, fast, and reliable protocol for in-house AAV vector production based on chloroform extraction. Current Protocols in Neuroscience, 93, e103 (2020). doi: 10.1002/cpns.103.

Tiklova K, Nolbrant S, Fiorenzano A, Björklund Å, Sharma Y, Heuer A, Gillberg L, Hoban D, Cardoso T, Adler AF, Virtele M, Lunden-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parm. ar M: Single cell transcriptomics identifies stem cell-derived graft composition in a model of Parkinson’s disease. Nature Communications volume 11, Article number: 2434 (2020) 10.1038/s41467-020-16225-5.

Precious SV*, Smith GA*, Heuer A, Jaeger I, Lane EL, Dunnett SB, Li M, Kelly CM, Rosser AE: Dopaminergic progenitors derived from epiblast stem cells function similarly to primary VM-derived progenitors when transplanted into a Parkinson's disease model.  Frontiers in Neuroscience and Neurodegeneration.

* Joined 1st author


Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, Frostner EÅ, Elmer E, Hyttel P, Cappelletti G, Zini M, Goldwurm S, Roybon L: Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun 7, 208 (2019). doi:10.1186/s40478-019-0863-7

Espa E, Clemensson EKH, Luk KC, Heuer A, Björklund T, Cenci MA:Seeding of protein aggregation causes cognitive impairment in rat model of cortical synucleinopathy. Movement Disorders.

Tiklová K, Nolbrant S, Fiorenzano A, Björklund AK, Sharma Y, Heuer A, Gillberg L, Hoban DB, Cardoso T, Adler AF, Birtele M, Lundén-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parmar M: Single Cell Gene Expression Analysis Reveals Human Stem Cell-Derived Graft Composition in a Cell Therapy Model of Parkinson’s Disease. BioRxiv.



Yhnell E & Heuer A: Automated operant assessments of Huntington’s disease mouse models. In Dunnett SB, Precious SP and Rosser AE (eds.), Methods in Molecular Biology: Huntington’s disease. Springer. June 2018. doi: 10.1007/978-1-4939-7825-0_8


Davidsson M, Diaz-Fernandez P, Torroba M, Schwich OD, Aldrin-Kirk P, Quintino L, Heuer A, Wang G, Lundberg C, Björklund T: Molecular barcoding of viral vectors enables quantification and mapping of RNA-editing events and optimization of mRNA trans-splicing. RNA. doi: 10.1261/rna.063925.117


Aldrin-Kirk P, Heuer A, Rylander D, Davidsson M, Mattsson B, Bjorklund T: Chemogenetic modulation of striatal cholinergic interneurons proives insight into the interplay with dopamine in a rat model of Parkinson’s disease. Neurobiology of Disease. 109: 148-162; 2018.


Burmeister JJ, Pomerleau F, Quintero JE, Huettl P, Ai Y, Jakobsson J, Lundblad M, Heuer A, Slevin JT, Gerhardt GA: In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays. In Parrot S and Denoroy L (eds.), Biochemical approachesfor glutamatergic neurotransmission: Neuromethods: Volume 130.

doi: 10.1007/978-1-4939-7228-9_11




Avaliani N, Pfisterer U, Heuer A, Parmar M, Kokaia M, Andersson M: Converted human neurons mature in adult rat hippocampus. Stem Cells International. 2017: 9p; 2017.


Lehnen D, Barral S, Cardoso T, Grealish S, Heuer A, Kirkeby A, Kollet J, Cremer H, Parmar M, Bosio A, Knoebel S: IAP-based cell sorting results in homogeneous transplantable dopaminergic precursor cells derived from human pluripotent stem cells. Stem Cell Reports. 9: 1-14; 2017.


Noldbrant S, Heuer A, Parmar M, Kirkeby A: Generation of high-purity ventral midbrain dopaminergic progenitors for in vivo maturation and intracerebral transplantation. Nature Protocols, 12(9): 1962-1979; 2017. doi: 10.1038/nprot.2017.078


Kirkeby A, Nolbrant S, Tiklova K, Heuer A, Kee N, Cardoso T, Rylander D, Lelos M, Rifes P, Dunnett SB, Perlmann T, Parmar M: Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based cell therapy for Parkinson’s disease. Cell Stem Cell, 20: 1-14; 2017. doi: 10.1016/j.stem.2016.09.004




Heuer A, Kirkeby A, Pfisterer U, Jonsson M, Parmar M: hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Experimental Neurology, 282: 78-85; 2016. doi: 10.1016/j.expneurol.2016.05.027


Aldrin-Kirk P*, Heuer A*, Mattsson B, Wang G, Lundblad M, Parmar M, Björklund T: DREADD modulation of transplanted DA neurons reveals a novel parkinsonian dyskinesia mechanism mediated by the serotonin 5-HT6 receptor. Neuron, 90: 1-14; 2016. doi: 10.1016/j.neuron.2016.04.017

* Joined 1st author




Grealish S*, Heuer A*, Cardoso T, Kirkeby A, Jonsson M, Johansson J, Björklund A, Jakobsson J, Parmar M: Monosynaptic tracing using modified rabies virus reveal early and extensive circuit integration of human embryonic stem cell-derived neurons. Stem Cell Reports, 4(6): 975-983; 2015.

* Joined 1st author


Arber C, Precious SV, Cambray S, Risner-Janiczek JR, Kelly C, Noakes Z, Fjordorova M, Heuer A, Ungless MA, Rodriguez TA, Rosser AE, Dunnett SB, Li M: Activin A directs striatal projection neuron differentiation of human pluripotent stem cells. Development, 124: 1375-1386; 2015. doi: 10.1242/dev.117093  


Cerovic M, Bagetta V, Pendolino V, Ghiglieri V, Fasano S, Morella I, Hardingham N, Heuer A, Papale A, Marchisella F, Giampà C, Calabresi P, Picconi B, Brambilla R.: Derangement of Ras-GuanineNucleotide-Releasing Factor 1 (Ras-GRF1) and Extracellular Signal-Regulated Kinase (ERK) Dependent Striatal Plasticity in L-DOPA-Induced Dyskinesia. Biological Psychiatry,77(2): 106-15; 2015.




Grealish S, Diguet E, Kirkeby A, Mattson B, Heuer A, Bramoulle Y, Van Camp N, Perrier AL, Hantraye P, Bjorklund A, Parmar M.: Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson’s disease. Cell Stem Cell, 15(5): 653-65; 2014.




Heuer A, Vinh NN, Dunnett SB: Behavioural recovery on simple and complex tasks by means of cell replacement therapy in mice. European Journal of Neuroscience, 37(10): 2691-704; 2013.


Heuer A, Lelos MJ, Kelly CM, Torres EM, Dunnett SB: Dopamine-rich grafts alleviate deficits in contralateral response space induced by extensive dopamine depletion in rats. Experimental Neurology, 247: 485-95; 2013.


Heuer A, Dunnett SB: Characterisation of spatial neglect induced by unilateral 6-OHDA lesions on a choice reaction time task in rats. Behavioural Brain Research, 237: 215-22; 2013.


Smith GA, Heuer A, Klein A, Vinh NN, Dunnett SB, Lane EL: Amphetamine-induced dyskinesia inthe transplanted hemi-parkinsonian mouse. Journal of Parkinson’s disease, 2: 107-113; 2013. DOI: 10.3233/JPD-2012-12102




Heuer A, Smith GA, Dunnett SB: Comparison of 6-hydroxydopamine lesions of the substantia nigra and the medial forebrain bundle on a lateralised choice reaction time task in mice. European Journal of Neuroscience, 37(2): 294-302; 2012.


Heuer A, Dunnett SB: Unilateral 6-OHDA lesions induce lateralised deficits in a ‘Skinner box’ operant choice reaction time task in rats. Journal of Parkinson’s disease, 2(4): 309-20; 2012. doi: 10.3233/JPD-2012-012133


Heuer A*, Smith GA*, Lelos MJ, Lane EL, Dunnett SB: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice I: motor impairments identify the extent of dopamine depletion at three different lesion sites. Behavioural Brain Research, 228(1): 30-43; 2012. doi: 10.1016/j.bbr.2011.11.027

* Joined 1st author


Smith GA* & Heuer A*, Dunnett SB, Lane EL: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice II: predicting l-DOPA-induced dyskinesia. Behavioural Brian Research, 226(1): 281-92; 2012. doi: 10.1016/j.bbr.2011.09.025

* Joined 1st author


Torres EM, Lane EL, Heuer A, Smith GA, Murphy E, Dunnett SB: Increased efficacy of the 6-hydroxydopamine lesion of the median forebrain bundle in small rats, by modification of the stereotaxic coordinates. Journal of Neuroscience Methods, 200(1):29-35; 2011.


Brooks SP, Janghra N, Higgs GV, Bayram-Weston Z, Heuer A, Jones L, Dunnett SB.: Selective cognitive impairment in the YAC128 Huntington’s disease mouse. Brain Research Bulletin, 88(2-1): 121-9; 2012.


Dunnett SB, Heuer A, Lelos M, Brooks SP, Rosser AE: Bilateral striatal lesions disrupt performance in an operant delayed reinforcement task in rats. Brain Research Bulletin, 88(2-3): 251-60); 2012. doi: 10.1016/j.brainresbull.2011.04.002




Smith GA & Heuer A: The 6-OHDA Lesioned Mouse Model of Parkinson’s Disease. In Lane EL and Dunnett SB (eds.), Animal Models of Movement Disorders, Volume I. Springer Protocols, Humana Press, New York (2011). pp. 281-287.