PUBLICATIONS

2024

Rifes P, Kajtez J, Rågåard Christiansen J, Schörling A, Singh Rathore G, Wolf DA, Heuer A, Kirkeby A: Forced LMX1A expression induces dorsal neural fates and disrupts patterning of human embryonic stem cells into ventral midgrain dopaminergic neurons. Stem Cell Reports. 2024.

https://doi.org/10.1016/j.stemcr.2024.04.010

2023

Anwer DM*, Gubinelli F*, Kurt YA, Sarauskyte L, Jacobs F, Venuti C, Sandoval IM, Yang Y, Stancati J, Mazzocchi M, Brandi E, O'Keeffe G, Steece-Collier K, Li J-Y, Deierborg T, Manfredsson FP, Davidsson M, Heuer A: A comparison of machine learning approaches for the quantification of microglial cells in the brain of mice, rats and non-human primates. Plos One. 2023.

https://doi.org/10.1371/journal.pone.0284480

* Joined 1st author

Rajova J, Davidsson M, Hartnor M, Aldrin-Kirk P, Cardoso T, Nolbrant S, Mollbrink A, Storm P, Heuer A, Parmar M, Björklund T: Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. Molecular Therapy - Methods & Clinical Development. 2023.

https://doi.org/10.1016/j.omtm.2023.04.008

Canals I, Comella-Bolla A, Cepeda-Prado E, Avaliani N, Crowe JA, Oburoglu L, Bruzelius A, King N, Pajares MA, Perez-Sala D, Heuer A, Rylander-Ottosson D, Soriano J, Ahlenius H: Astrocyte dysfunction and neuronal network hyperactivity in a CRISPR engineered pluripotent stem cell model of frontotemporal dementia. Brain Communications. 2023.

https://academic.oup.com/braincomms/advance-article/doi/10.1093/braincomms/fcad158/7170310

Gubinelli F, Sarauskyte L, Venuti C, Kulacz I, Cazzolla G, Negrini M, Anwer D, Vecchio I, Jacobs F, Manfredsson FP, Davidsson M, Heuer A: Characterisation of functional deficits induced by AAV overexpression of alpha-synuclein in rats. Current Research in Neurobiology 4, 100065. 2023. https://doi.org/10.1016/j.crneur.2022.100065

2022

Quintino L*, Gubinelli F*, Arvidsson E, Davidsson M, Lundberg C, Heuer A: Automated quantification of neuronal swellings in a preclinical rodent model of Parkinson’s disease detects region-specific changes in pathology. Journal of Neuroscience Methods. 2022. doi: 10.1016/j.jneumeth.2022.109640

* Joined 1st author

Gubinelli F, Cazzolla G, Negrini M, Kulacz I, Mehrdadian A, Tomasello G, Sarauskyte L, Venuti C, Jacobs F, Manfredsson FP, Davidsson M, Heuer A: Lateralized deficits after unilateral AAV-vector based overexpression of alphasynuclein in the midbrain of rats on drug-free behavioural tests. Behavioural Brain Research. 2022. https://doi.org/10.1016/j.bbr.2022.113887

Negrini M, Tomasello G, Davidsson M, Fenyi A, Adant C, Hauser S, Espa E, Gubinelli F, Manfredsson FP, Melki R, Heuer A: Sequential or simultaneous injection of preformed fibrils and AAV overexpression of alpha-synuclein are equipotent in producing relevant pathology and behavioural deficits.

Journal of Parkinson's Disease. 2022. https://content.iospress.com/articles/journal-of-parkinsons-disease/jpd212555

Aldrin-Krik P, Akerblom M, Cardoso T, Nolbrant S, Adler A, Liu X, Heuer A, Davidsson M, Parmar M, Björklund T: A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants. Stem Cell Reports. 2021. 10.1016/j.stemcr.2021.11.014

Davidsson M, Heuer A: Small scale adeno-associated virus-vector prodicion for preclinical gene delivery based on chloroform precipitation. Neural Regeneration Research 17(1):99-100. https://doi.org/10.4103/1673-5374.314309

2021

Heuer A: Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods.Methods in Molecular Biology 2352:201-226. 10.1007/978-1-0716-1601-7_14

Rylander Ottosson D, Grade S, and Heuer A: Editorial: Regeneration and Brain Repair. Frontiers in Cellular Neuroscience, (2021). doi: 10.3389/fncel.2021.687992

Frontiers special issue edited:

https://www.frontiersin.org/research-topics/9641/regeneration-and-brain-repair

2020

Davidsson M, Negrini M, Hauser S, Svanbergsson A, Lockowandt M, Tomasello G, Manfredsson FP, Heuer A: A comparison of AAV-vector production methods for gene therapy and preclinical assessment. Scientific Reports, (2020) 10:21532. https://www.nature.com/articles/s41598-020-78521-w

Negrini M, Wang G, Heuer A, Björklund T, & Davidsson M: AAV production everywhere: A simple, fast, and reliable protocol for in-house AAV vector production based on chloroform extraction. Current Protocols in Neuroscience, 93, e103 (2020). doi: 10.1002/cpns.103.

Tiklova K, Nolbrant S, Fiorenzano A, Björklund Å, Sharma Y, Heuer A, Gillberg L, Hoban D, Cardoso T, Adler AF, Virtele M, Lunden-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parm. ar M: Single cell transcriptomics identifies stem cell-derived graft composition in a model of Parkinson’s disease. Nature Communications volume 11, Article number: 2434 (2020) 10.1038/s41467-020-16225-5.

Precious SV*, Smith GA*, Heuer A, Jaeger I, Lane EL, Dunnett SB, Li M, Kelly CM, Rosser AE: Dopaminergic progenitors derived from epiblast stem cells function similarly to primary VM-derived progenitors when transplanted into a Parkinson's disease model.  Frontiers in Neuroscience and Neurodegeneration. https://doi.org/10.3389/fnins.2020.00312.

* Joined 1st author

2019

Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, Frostner EÅ, Elmer E, Hyttel P, Cappelletti G, Zini M, Goldwurm S, Roybon L: Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun 7, 208 (2019). doi:10.1186/s40478-019-0863-7

Espa E, Clemensson EKH, Luk KC, Heuer A, Björklund T, Cenci MA:Seeding of protein aggregation causes cognitive impairment in rat model of cortical synucleinopathy. Movement Disorders. https://doi.org/10.1002/mds.27810

Tiklová K, Nolbrant S, Fiorenzano A, Björklund AK, Sharma Y, Heuer A, Gillberg L, Hoban DB, Cardoso T, Adler AF, Birtele M, Lundén-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parmar M: Single Cell Gene Expression Analysis Reveals Human Stem Cell-Derived Graft Composition in a Cell Therapy Model of Parkinson’s Disease. BioRxiv. https://doi.org/10.1101/720870

2018

Yhnell E & Heuer A: Automated operant assessments of Huntington’s disease mouse models. In Dunnett SB, Precious SP and Rosser AE (eds.), Methods in Molecular Biology: Huntington’s disease. Springer. June 2018. doi: 10.1007/978-1-4939-7825-0_8

Davidsson M, Diaz-Fernandez P, Torroba M, Schwich OD, Aldrin-Kirk P, Quintino L, Heuer A, Wang G, Lundberg C, Björklund T: Molecular barcoding of viral vectors enables quantification and mapping of RNA-editing events and optimization of mRNA trans-splicing. RNA. doi: 10.1261/rna.063925.117

Aldrin-Kirk P, Heuer A, Rylander D, Davidsson M, Mattsson B, Bjorklund T: Chemogenetic modulation of striatal cholinergic interneurons proives insight into the interplay with dopamine in a rat model of Parkinson’s disease. Neurobiology of Disease. 109: 148-162; 2018. http://dx.doi.org/10.1016/j.nbd.2017.10.010

Burmeister JJ, Pomerleau F, Quintero JE, Huettl P, Ai Y, Jakobsson J, Lundblad M, Heuer A, Slevin JT, Gerhardt GA: In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays. In Parrot S and Denoroy L (eds.), Biochemical approachesfor glutamatergic neurotransmission: Neuromethods: Volume 130.

doi: 10.1007/978-1-4939-7228-9_11

2017

Avaliani N, Pfisterer U, Heuer A, Parmar M, Kokaia M, Andersson M: Converted human neurons mature in adult rat hippocampus. Stem Cells International. 2017: 9p; 2017. https://doi.org/10.1155/2017/5718608

Lehnen D, Barral S, Cardoso T, Grealish S, Heuer A, Kirkeby A, Kollet J, Cremer H, Parmar M, Bosio A, Knoebel S: IAP-based cell sorting results in homogeneous transplantable dopaminergic precursor cells derived from human pluripotent stem cells. Stem Cell Reports. 9: 1-14; 2017. https://doi.org/10.1016/j.stemcr.2017.08.016

Noldbrant S, Heuer A, Parmar M, Kirkeby A: Generation of high-purity ventral midbrain dopaminergic progenitors for in vivo maturation and intracerebral transplantation. Nature Protocols, 12(9): 1962-1979; 2017. doi: 10.1038/nprot.2017.078

Kirkeby A, Nolbrant S, Tiklova K, Heuer A, Kee N, Cardoso T, Rylander D, Lelos M, Rifes P, Dunnett SB, Perlmann T, Parmar M: Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based cell therapy for Parkinson’s disease. Cell Stem Cell, 20: 1-14; 2017. doi: 10.1016/j.stem.2016.09.004

2016

Heuer A, Kirkeby A, Pfisterer U, Jonsson M, Parmar M: hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Experimental Neurology, 282: 78-85; 2016. doi: 10.1016/j.expneurol.2016.05.027

Aldrin-Kirk P*, Heuer A*, Mattsson B, Wang G, Lundblad M, Parmar M, Björklund T: DREADD modulation of transplanted DA neurons reveals a novel parkinsonian dyskinesia mechanism mediated by the serotonin 5-HT6 receptor. Neuron, 90: 1-14; 2016. doi: 10.1016/j.neuron.2016.04.017

* Joined 1st author

2015

Grealish S*, Heuer A*, Cardoso T, Kirkeby A, Jonsson M, Johansson J, Björklund A, Jakobsson J, Parmar M: Monosynaptic tracing using modified rabies virus reveal early and extensive circuit integration of human embryonic stem cell-derived neurons. Stem Cell Reports, 4(6): 975-983; 2015. doi.org/10.1016/j.stemcr.2015.04.011

* Joined 1st author

Arber C, Precious SV, Cambray S, Risner-Janiczek JR, Kelly C, Noakes Z, Fjordorova M, Heuer A, Ungless MA, Rodriguez TA, Rosser AE, Dunnett SB, Li M: Activin A directs striatal projection neuron differentiation of human pluripotent stem cells. Development, 124: 1375-1386; 2015. doi: 10.1242/dev.117093  

Cerovic M, Bagetta V, Pendolino V, Ghiglieri V, Fasano S, Morella I, Hardingham N, Heuer A, Papale A, Marchisella F, Giampà C, Calabresi P, Picconi B, Brambilla R.: Derangement of Ras-GuanineNucleotide-Releasing Factor 1 (Ras-GRF1) and Extracellular Signal-Regulated Kinase (ERK) Dependent Striatal Plasticity in L-DOPA-Induced Dyskinesia. Biological Psychiatry,77(2): 106-15; 2015. doi.org/10.1016/j.biopsych.2014.04.002

2014

Grealish S, Diguet E, Kirkeby A, Mattson B, Heuer A, Bramoulle Y, Van Camp N, Perrier AL, Hantraye P, Bjorklund A, Parmar M.: Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson’s disease. Cell Stem Cell, 15(5): 653-65; 2014. doi.org/10.1016/j.stem.2014.09.017

2013

Heuer A, Vinh NN, Dunnett SB: Behavioural recovery on simple and complex tasks by means of cell replacement therapy in mice. European Journal of Neuroscience, 37(10): 2691-704; 2013. doi.org/10.1111/ejn.12150

Heuer A, Lelos MJ, Kelly CM, Torres EM, Dunnett SB: Dopamine-rich grafts alleviate deficits in contralateral response space induced by extensive dopamine depletion in rats. Experimental Neurology, 247: 485-95; 2013. doi.org/10.1016/j.expneurol.2013.01.020

Heuer A, Dunnett SB: Characterisation of spatial neglect induced by unilateral 6-OHDA lesions on a choice reaction time task in rats. Behavioural Brain Research, 237: 215-22; 2013. doi.org/10.1016/j.bbr.2012.09.038

Smith GA, Heuer A, Klein A, Vinh NN, Dunnett SB, Lane EL: Amphetamine-induced dyskinesia inthe transplanted hemi-parkinsonian mouse. Journal of Parkinson’s disease, 2: 107-113; 2013. DOI: 10.3233/JPD-2012-12102

2012

Heuer A, Smith GA, Dunnett SB: Comparison of 6-hydroxydopamine lesions of the substantia nigra and the medial forebrain bundle on a lateralised choice reaction time task in mice. European Journal of Neuroscience, 37(2): 294-302; 2012. doi.org/10.1111/ejn.12036

Heuer A, Dunnett SB: Unilateral 6-OHDA lesions induce lateralised deficits in a ‘Skinner box’ operant choice reaction time task in rats. Journal of Parkinson’s disease, 2(4): 309-20; 2012. doi: 10.3233/JPD-2012-012133

Heuer A*, Smith GA*, Lelos MJ, Lane EL, Dunnett SB: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice I: motor impairments identify the extent of dopamine depletion at three different lesion sites. Behavioural Brain Research, 228(1): 30-43; 2012. doi: 10.1016/j.bbr.2011.11.027

* Joined 1st author

Smith GA* & Heuer A*, Dunnett SB, Lane EL: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice II: predicting l-DOPA-induced dyskinesia. Behavioural Brian Research, 226(1): 281-92; 2012. doi: 10.1016/j.bbr.2011.09.025

* Joined 1st author

Torres EM, Lane EL, Heuer A, Smith GA, Murphy E, Dunnett SB: Increased efficacy of the 6-hydroxydopamine lesion of the median forebrain bundle in small rats, by modification of the stereotaxic coordinates. Journal of Neuroscience Methods, 200(1):29-35; 2011. doi.org/10.1016/j.jneumeth.2011.06.012

Brooks SP, Janghra N, Higgs GV, Bayram-Weston Z, Heuer A, Jones L, Dunnett SB.: Selective cognitive impairment in the YAC128 Huntington’s disease mouse. Brain Research Bulletin, 88(2-1): 121-9; 2012. doi.org/10.1016/j.brainresbull.2011.05.010

Dunnett SB, Heuer A, Lelos M, Brooks SP, Rosser AE: Bilateral striatal lesions disrupt performance in an operant delayed reinforcement task in rats. Brain Research Bulletin, 88(2-3): 251-60); 2012. doi: 10.1016/j.brainresbull.2011.04.002

2011

Smith GA & Heuer A: The 6-OHDA Lesioned Mouse Model of Parkinson’s Disease. In Lane EL and Dunnett SB (eds.), Animal Models of Movement Disorders, Volume I. Springer Protocols, Humana Press, New York (2011). pp. 281-287.