PUBLICATIONS

2021


Davidsson M, Heuer A: Small scale AAV-vector production for preclinical gene delivery based on chloroform precipitation. Neural Regeneration Research, (2021). accepted for publication.


2020


Davidsson M, Negrini M, Hauser S, Svanbergsson A, Lockowandt M, Tomasello G, Manfredsson FP, Heuer AA comparison of AAV-vector production methods for gene therapy and preclinical assessment. Scientific Reports, (2020) 10:21532. https://www.nature.com/articles/s41598-020-78521-w


Negrini M, Wang G, Heuer A, Björklund T, & Davidsson M: AAV production everywhere: A simple, fast, and reliable protocol for in-house AAV vector production based on chloroform extraction. Current Protocols in Neuroscience, 93, e103 (2020). doi: 10.1002/cpns.103.


Tiklova K, Nolbrant S, Fiorenzano A, Björklund Å, Sharma Y, Heuer A, Gillberg L, Hoban D, Cardoso T, Adler AF, Virtele M, Lunden-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parm. ar M: Single cell transcriptomics identifies stem cell-derived graft composition in a model of Parkinson’s disease. Nature Communications volume 11, Article number: 2434 (2020) 10.1038/s41467-020-16225-5.


Precious SV*, Smith GA*, Heuer A, Jaeger I, Lane EL, Dunnett SB, Li M, Kelly CM, Rosser AE: Dopaminergic progenitors derived from epiblast stem cells function similarly to primary VM-derived progenitors when transplanted into a Parkinson's disease model.  Frontiers in Neuroscience and Neurodegeneration. https://doi.org/10.3389/fnins.2020.00312.


2019


Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, Frostner EÅ, Elmer E, Hyttel P, Cappelletti G, Zini M, Goldwurm S, Roybon L: Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun 7, 208 (2019). doi:10.1186/s40478-019-0863-7


Espa E, Clemensson EKH, Luk KC, Heuer A, Björklund T, Cenci MA:Seeding of protein aggregation causes cognitive impairment in rat model of cortical synucleinopathy. Movement Disorders. https://doi.org/10.1002/mds.27810


Tiklová K, Nolbrant S, Fiorenzano A, Björklund AK, Sharma Y, Heuer A, Gillberg L, Hoban DB, Cardoso T, Adler AF, Birtele M, Lundén-Miguel H, Volakakis N, Kirkeby A, Perlmann T, Parmar M: Single Cell Gene Expression Analysis Reveals Human Stem Cell-Derived Graft Composition in a Cell Therapy Model of Parkinson’s Disease. BioRxiv. https://doi.org/10.1101/720870


2018

 

Yhnell E & Heuer A: Automated operant assessments of Huntington’s disease mouse models. In Dunnett SB, Precious SP and Rosser AE (eds.), Methods in Molecular Biology: Huntington’s disease. Springer. June 2018. doi: 10.1007/978-1-4939-7825-0_8

 

Davidsson M, Diaz-Fernandez P, Torroba M, Schwich OD, Aldrin-Kirk P, Quintino L, Heuer A, Wang G, Lundberg C, Björklund T: Molecular barcoding of viral vectors enables quantification and mapping of RNA-editing events and optimization of mRNA trans-splicing. RNA. doi: 10.1261/rna.063925.117

 

Aldrin-Kirk P, Heuer A, Rylander D, Davidsson M, Mattsson B, Bjorklund T: Chemogenetic modulation of striatal cholinergic interneurons proives insight into the interplay with dopamine in a rat model of Parkinson’s disease. Neurobiology of Disease. 109: 148-162; 2018. http://dx.doi.org/10.1016/j.nbd.2017.10.010

 

Burmeister JJ, Pomerleau F, Quintero JE, Huettl P, Ai Y, Jakobsson J, Lundblad M, Heuer A, Slevin JT, Gerhardt GA: In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays. In Parrot S and Denoroy L (eds.), Biochemical approachesfor glutamatergic neurotransmission: Neuromethods: Volume 130.

doi: 10.1007/978-1-4939-7228-9_11

 

2017

 

Avaliani N, Pfisterer U, Heuer A, Parmar M, Kokaia M, Andersson M: Converted human neurons mature in adult rat hippocampus. Stem Cells International. 2017: 9p; 2017. https://doi.org/10.1155/2017/5718608

 

Lehnen D, Barral S, Cardoso T, Grealish S, Heuer A, Kirkeby A, Kollet J, Cremer H, Parmar M, Bosio A, Knoebel S: IAP-based cell sorting results in homogeneous transplantable dopaminergic precursor cells derived from human pluripotent stem cells. Stem Cell Reports. 9: 1-14; 2017. https://doi.org/10.1016/j.stemcr.2017.08.016

 

Noldbrant S, Heuer A, Parmar M, Kirkeby A: Generation of high-purity ventral midbrain dopaminergic progenitors for in vivo maturation and intracerebral transplantation. Nature Protocols, 12(9): 1962-1979; 2017. doi: 10.1038/nprot.2017.078

 

Kirkeby A, Nolbrant S, Tiklova K, Heuer A, Kee N, Cardoso T, Rylander D, Lelos M, Rifes P, Dunnett SB, Perlmann T, Parmar M: Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based cell therapy for Parkinson’s disease. Cell Stem Cell, 20: 1-14; 2017. doi: 10.1016/j.stem.2016.09.004

 

2016

 

Heuer A, Kirkeby A, Pfisterer U, Jonsson M, Parmar M: hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Experimental Neurology, 282: 78-85; 2016. doi: 10.1016/j.expneurol.2016.05.027

 

Aldrin-Kirk P*, Heuer A*, Mattsson B, Wang G, Lundblad M, Parmar M, Björklund T: DREADD modulation of transplanted DA neurons reveals a novel parkinsonian dyskinesia mechanism mediated by the serotonin 5-HT6 receptor. Neuron, 90: 1-14; 2016. doi: 10.1016/j.neuron.2016.04.017

 

2015

 

Grealish S*, Heuer A*, Cardoso T, Kirkeby A, Jonsson M, Johansson J, Björklund A, Jakobsson J, Parmar M: Monosynaptic tracing using modified rabies virus reveal early and extensive circuit integration of human embryonic stem cell-derived neurons. Stem Cell Reports, 4(6): 975-983; 2015. doi.org/10.1016/j.stemcr.2015.04.011

 

Arber C, Precious SV, Cambray S, Risner-Janiczek JR, Kelly C, Noakes Z, Fjordorova M, Heuer A, Ungless MA, Rodriguez TA, Rosser AE, Dunnett SB, Li M: Activin A directs striatal projection neuron differentiation of human pluripotent stem cells. Development, 124: 1375-1386; 2015. doi: 10.1242/dev.117093  

 

Cerovic M, Bagetta V, Pendolino V, Ghiglieri V, Fasano S, Morella I, Hardingham N, Heuer A, Papale A, Marchisella F, Giampà C, Calabresi P, Picconi B, Brambilla R.: Derangement of Ras-GuanineNucleotide-Releasing Factor 1 (Ras-GRF1) and Extracellular Signal-Regulated Kinase (ERK) Dependent Striatal Plasticity in L-DOPA-Induced Dyskinesia. Biological Psychiatry,77(2): 106-15; 2015. doi.org/10.1016/j.biopsych.2014.04.002

 

2014

 

Grealish S, Diguet E, Kirkeby A, Mattson B, Heuer A, Bramoulle Y, Van Camp N, Perrier AL, Hantraye P, Bjorklund A, Parmar M.: Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson’s disease. Cell Stem Cell, 15(5): 653-65; 2014. doi.org/10.1016/j.stem.2014.09.017

 

2013

 

Heuer A, Vinh NN, Dunnett SB: Behavioural recovery on simple and complex tasks by means of cell replacement therapy in mice. European Journal of Neuroscience, 37(10): 2691-704; 2013. doi.org/10.1111/ejn.12150

 

Heuer A, Lelos MJ, Kelly CM, Torres EM, Dunnett SB: Dopamine-rich grafts alleviate deficits in contralateral response space induced by extensive dopamine depletion in rats. Experimental Neurology, 247: 485-95; 2013. doi.org/10.1016/j.expneurol.2013.01.020

 

Heuer A, Dunnett SB: Characterisation of spatial neglect induced by unilateral 6-OHDA lesions on a choice reaction time task in rats. Behavioural Brain Research, 237: 215-22; 2013. doi.org/10.1016/j.bbr.2012.09.038

 

Smith GA, Heuer A, Klein A, Vinh NN, Dunnett SB, Lane EL: Amphetamine-induced dyskinesia inthe transplanted hemi-parkinsonian mouse. Journal of Parkinson’s disease, 2: 107-113; 2013. DOI: 10.3233/JPD-2012-12102

 

2012

 

Heuer A, Smith GA, Dunnett SB: Comparison of 6-hydroxydopamine lesions of the substantia nigra and the medial forebrain bundle on a lateralised choice reaction time task in mice. European Journal of Neuroscience, 37(2): 294-302; 2012. doi.org/10.1111/ejn.12036

 

Heuer A, Dunnett SB: Unilateral 6-OHDA lesions induce lateralised deficits in a ‘Skinner box’ operant choice reaction time task in rats. Journal of Parkinson’s disease, 2(4): 309-20; 2012. doi: 10.3233/JPD-2012-012133

 

Heuer A*, Smith GA*, Lelos MJ, Lane EL, Dunnett SB: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice I: motor impairments identify the extent of dopamine depletion at three different lesion sites. Behavioural Brain Research, 228(1): 30-43; 2012. doi: 10.1016/j.bbr.2011.11.027

 

Smith GA* & Heuer A*, Dunnett SB, Lane EL: Unilateral nigrostriatal 6-hydroxydopamine lesions in mice II: predicting l-DOPA-induced dyskinesia. Behavioural Brian Research, 226(1): 281-92; 2012. doi: 10.1016/j.bbr.2011.09.025

 

Torres EM, Lane EL, Heuer A, Smith GA, Murphy E, Dunnett SB: Increased efficacy of the 6-hydroxydopamine lesion of the median forebrain bundle in small rats, by modification of the stereotaxic coordinates. Journal of Neuroscience Methods, 200(1):29-35; 2011. doi.org/10.1016/j.jneumeth.2011.06.012

 

Brooks SP, Janghra N, Higgs GV, Bayram-Weston Z, Heuer A, Jones L, Dunnett SB.: Selective cognitive impairment in the YAC128 Huntington’s disease mouse. Brain Research Bulletin, 88(2-1): 121-9; 2012. doi.org/10.1016/j.brainresbull.2011.05.010

 

Dunnett SB, Heuer A, Lelos M, Brooks SP, Rosser AE: Bilateral striatal lesions disrupt performance in an operant delayed reinforcement task in rats. Brain Research Bulletin, 88(2-3): 251-60); 2012. doi: 10.1016/j.brainresbull.2011.04.002

 

2011

 

Smith GA & Heuer A: The 6-OHDA Lesioned Mouse Model of Parkinson’s Disease. In Lane EL and Dunnett SB (eds.), Animal Models of Movement Disorders, Volume I. Springer Protocols, Humana Press, New York (2011). pp. 281-287.